secvorti.blogg.se - Logger pro absorbance

#Logger pro absorbance drivers#
#Logger pro absorbance full#
#Logger pro absorbance software#
#Logger pro absorbance plus#

If the spectrum maxes out (flat and wide peaks), increase the distance between the light source and the tip of the optical fiber cable.

Note: If you are conducting a flame test, place the tip of the optical fiber no closer than 4–5 cm from the flame.

Aim the tip of the optical fiber cable at a light source.

#Logger pro absorbance plus#

SpectroVis Plus measures intensity in relative units scaled from 0 to 1.

If you have not already done so, choose Change Units -> Spectrometer ->Intensity from the Experiment menu.

Or, if measuring the luminescence of a solution, insert the cuvette.

Insert the SpectroVis Optical Fiber into the SpectroVis Plus, lining up the white triangles.

SpectroVis Optical Fiber for emission or fluorescence. Select the unit or data type you wish to measure To switch from absorbance to %transmittance, Fluorescence, or Intensity go to Experiment in the menu toolbar and select Change Units -> Spectrometer.

Click Curve Fit, to calculate a function for your data.

You may click to end the data collection early.

Click Collect to begin data collection.Place the cuvette in the cuvette slot of SpectroVis Plus. Mix the reactants then transfer ~2 mL of the reaction mixture to a cuvette.See “Changing the Settings in Logger Pro 3” section below. Note: If data are being collected more slowly than you wish, you may need to reduce the Samples to Average value in the Spectrometer Dialog Box. If you wish to change the data collection parameters for your experiment, choose Data Collection from the Experiment menu and make the necessary changes. The default is set to collect data for 200 seconds.See Step 3 in the previous section for instructions. You may wish to select a different wavelength. The wavelength with the maximum value from the spectrum (λ max) will be automatically selected. Select Absorbance (or %T or Fluor.) vs.Click the Configure Spectrometer Data Collection button (a graph with a rainbow under the curve).The available wavelengths are given in the list of wavelength options. More than one wavelength can be chosen to be monitored. For both modes, the wavelength with the maximum absorbance value is chosen automatically to be monitored as a function of time or concentration. Choose the collection mode you wish to use after you’ve already generated a spectrum. To change modes, click the Configure Spectrometer Data Collection button (a graph with a rainbow under the curve).Then proceed with measuring the chosen value versus concentration or time. To use one of the other modes, begin by generating an absorbance spectrum as described above.There are two other modes besides Absorbance versus Wavelength:.Click the Collect button to generate a spectrum.

#Logger pro absorbance full#

To measure absorbance of the sample versus wavelength, fill a cuvette ¾ full of solution to be tested and place it in the spectrometer cuvette holder.

Calibration is not necessary in fluorescence or intensity modes.

Wait for a few seconds for the calibration to be completed, and then click OK.

When the warm up time is complete, click Finish Calibration.

Insert the blank cuvette into the cuvette holder.

You may wait longer than one minute for added stability.

The calibration dialog box will display the message: “Waiting …seconds for the lamp to warm up.” The minimum warm up time is one minute.

Prepare a blank cuvette with the solvent for your experiment.

To calibrate the device, choose Calibrate > Spectrometer from the Experiment menu.

To use the spectrometer in absorbance mode the spectrometer must be calibrated:.

Wavelength will be displayed of the visible spectrum in nanometers superimposed over the colors associated with those wavelengths.

#Logger pro absorbance software#

The software will automatically identify the spectrometer.

#Logger pro absorbance drivers#

Device drivers were included with the Logger Pro 3 software.

Note: Do not go online for device drivers.

The first time you connect a SpectroVis Plus, your computer may ask you a few questions.

Connect the spectrometer to the computer via a USB port.

Requires Logger Pro 3 (Version 3.8.2 or higher) when using with a computer. Go to for the complete instructions or see the hard copy in the prep room. These instructions are taken from the Specifications sheet provided by Vernier. Vernier Spectro-Vis Plus Spectrophotometer Overview